Please follow the links to get interactive access (Jmol) to the examples shown in the paper (Figure 3). Each example visualizes the splicing event as well as the superposition of spliced isoform and the protein from the other fold using Jmol. Additionally TOPS diagrams are shown to get a fast overview on the connectivity and topology of the examples shown. The correspondence of TOPS elements and the protein structures shown in Jmol may be explored interactively by clicking on an element in the TOPS diagram. The corresponding element in the structure will be colorized with the respective color. Please note that Jmol uses its own algorithm to annotate secondary structure elements in the cartoon view. Therefore those may differ from the secondary structure annotations (DSSP) used to create the TOPS diagrams which are more reliable than the Jmol assignments.

DNMT2_HUMAN (O14717, Isoform 6, Astral: d1g55a_, SCOP: c.66.1.26)

The spliced isoform superposes very well (TM-Score: 0.68) with d1gsoa2 from SCOP fold c.30.1.1. Topologically the two proteins are very similar except for a small strand-helix motif missing in the spliced isoform of d1g55a_. The strand of this small, missing motif has length 2, the helix a length of 4. According to their lengths, the secondary structure elements are very similar, some helices appear to be "broken" according to the DSSP structure annotations, but their combined lengths fit well to the lengths of the corresponding helix in the other structure.

Superposition of d1g55a_ and d1gsoa2 (Blue)	TOPS diagrams (click on elements to colorize on the structure)
	TOPS diagram of d1g55a_ (after splicing event) TOPS digram of d1gsoa2 Original coloring again
Visualization of the splicing event on d1g55a_

Download TOPS for d1g55a_
Download TOPS for d1gsoa2

AUHM_MOUSE (Q9JILZ3, Isoform 2, Astral: d1hzda_, SCOP: c.14.1.3)

The spliced isoform superposes well (TM-Score: 0.56) with d1vc1a_ from SCOP family c.13.2.1. Topologically the proteins are similar except for a small sheet of length 2 and a short helix of length 3 (missing in d1vc1a_) as well as one additional helix-strand motif which is missing in d1hzda_.

Superposition of d1hzda_ and d1vc1a_ (Blue)	TOPS diagrams (click on elements to colorize on the structure)
	TOPS diagram of d1hzda_ (after splicing event) TOPS digram of d1vc1a_ Original coloring again
Visualization of the splicing event on d1hzda_

Download TOPS for d1hzda_
Download TOPS for d1vc1a_

HER1_CAEEL (P34704, Astral: d1szha_, SCOP: a.226.1.1)

The spliced isoform superposes well with d1ni8a_ (SCOP: a.155.1.1, TM-Score: 0.49), which is known to form dimers and to be involved in DNA binding. Though, from a topological point of view, this example is not very interesting, it may represent an example, where small fragments left by larger scale splicing events may lead to stable fragments, for example by dimerization, which fulfill a function in the cell.

Superposition of d1szha_ and d1ni8a_ (Blue)	TOPS diagrams (click on elements to colorize on the structure)
	TOPS diagram of d1szha_ (after splicing event) TOPS digram of d1ni8a_ Original coloring again
Visualization of the splicing event on d1szha_

Download TOPS for d1szha_
Download TOPS for d1ni8a_

TIP30_HUMAN (Q9BUP3, Isoform 2, PDB: 2bka, SCOP: c.2.1.2)

This spliced isoform superposes well (TM-Score: 0.54) with d1gsoa2 from SCOP fold c.30.1.1 (see also the example of DNMT2_HUMAN). Topologically the two proteins are very similar except for two helices missing either at the C- or N-terminal end of either of the two proteins.

Superposition of 2bka and d1gsoa2 (blue)	TOPS diagrams (click on elements to colorize on the structure)
	TOPS diagram of 2bka (after splicing event) TOPS digram of d1gsoa2 Original coloring again
Visualization of the splicing event on 2bka

Download TOPS from 2bka
Download TOPS from d1gsoa2