iBRET – high confidence detection of protein-protein interactions in living cells

The quality of protein-protein interaction datasets covering the human interactome is limited with respect to coverage and accuracy. We present an automated informatics-aided and bioluminescence resonance energy transfer based (iBRET) method enabling high-throughput detection of interactions in living cells. Assay performance was benchmarked against a gold standard of five state-of-the-art methods resulting in significantly increased sensitivity at comparable specificity confirming its applicability for future genome-scale screens.

The iBRET software is freely available to all academic users